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Image Search Results
Journal: The Journal of Biological Chemistry
Article Title: Role of the Netrin-like Domain of Procollagen C-Proteinase Enhancer-1 in the Control of Metalloproteinase Activity
doi: 10.1074/jbc.M109.086447
Figure Lengend Snippet: Superstimulation of PCPE-1 enhancing activity by heparin. Effect of heparin (50 μg/ml; Sigma H-3393) on the activity of BMP-1 (10 n m ) on mini-procollagen III ( mini III , 400 n m ) in the presence and absence of PCPE-1His, its CUB1CUB2 or NTRt fragments, or a mixture of CUB1CUB2 and NTRt (all at 400 n m ). Incubations were carried out at 37 °C for 15 min. The migration positions of the released C-propeptide trimer ( CPIII ) and the N-terminal fragment ( Nter ) are indicated. Discontinuous SDS-PAGE (4–20% acrylamide in the separating gel) was carried out in nonreducing conditions, followed by staining with Coomassie Blue.
Article Snippet: The reaction products were then separated on
Techniques: Activity Assay, Migration, SDS Page, Staining